Eye irritation testing
OECD TG 492
For this test, 3D reconstructed human ocular models are exposed to the test material for 30 minutes (liquids) or 6 hours (solids). After exposure models are rinsed and post-soaked for 25 minutes. The viability of the models is subsequently assessed using the MTT test (colorimetric assay). If the remaining viability is > 60%, the test material is non-irritant.
Prediction Model
| Mean tissue viability (expressed as % of negative control) |
Prediction |
| > 60% | non-irritant (GHS no category, no labelling requirements) |
| < 60% | no prediction further testing required |
Note: OECD/OCDR TG No. 492 adopted the following classification in 2019: If the viability of the tissue treated with the test article is > 60.0% relative to negative control-treated tissue viability, the test article does not require classification and labelling (No Category) and no further testing is required. If the viability of the tissue treated with the test article is ≤ 60.0% relative to negative control-treated tissue viability, then no prediction can be made for the test article and further testing is required.