Eye irritation testing
OECD TG 492
For this test 3D human reconstructed ocular models are exposed to the test material for 30 minutes (liquids) or 6 hours (solids). After exposure models are rinsed and post-soaked for 25 minutes. Subsequently vitality of the models is assessed by MTT-test (colorimetric assay). If remaining vitality is > 60 %, the test material is non-irritant.
Prediction Model
| Mean tissue viability (expressed as % of negative control) |
Prediction |
| > 60 % | non-irritant (GHS no category, no labelling requirements) |
| < 60 % | no prediction further testing required |
Note: OECD/OCDR TG No. 492 adopted the following classification in 2019: If the test article-treated tissue viability is > 60.0% relative to negative control-treated tissue viability, the test article is not requiring classification and labelling (No Category) and no further testing is required. If the test article-treated tissue viability is ≤ 60.0% relative to negative control-treated tissue viability, then no prediction can be made for the test article and further testing is required.