Skin irritation testing

OECD TG 439

For this test, 3D reconstructed human skin models are exposed to the test material for 1 hour. Afterwards models are rinsed and incubated for 24 hours. The next day, supernatant can be collected for analysis of cytokines (e.g. IL-1α, optionally). Viability is subsequently assessed using the MTT test (colorimetric assay). If remaining viability is > 50% the test material is non-irritant.

Prediction Model

Mean tissue viability
(expressed as % of negative control)
in vivo prediction
EPI-200-SIT OECD TG No. 439
(adopted and updated 2019)
< 50% irritant (I)
(R38 or GHS category 2)
requiring classification and labelling (GHS Category 1 or 2)
> 50% non-irritant (NI) non-irritant to skin (GHS no category, no labelling requirements)

The Modified EpiDerm™ SIT allows discrimination to be made between irritants of category 2 and non-irritants. The test does not discriminate between non-mandatory subcategories of the UN GHS, i.e. it does not distinguish between GHS category 2 and category 3 irritants.

Schnellkontakt

Rufen Sie uns unter 0251 481637-0 an oder senden Sie eine Nachricht. Wir melden uns umgehend bei Ihnen.

Welche Daten im Einzelnen gespeichert werden und wer Zugriff auf diese hat, erfahren Sie in unserer Datenschutzerklärung.

Ich stimme der Speicherung und Nutzung meiner Daten laut Datenschutzerklärung zu und bin mit der Verarbeitung meiner Daten innerhalb der Dermatest GmbH für die Beantwortung meiner Kontakt- oder Informationsanfrage einverstanden.

Meine Einwilligungen kann ich jederzeit für die Zukunft widerrufen.

Quick request

Call us on 0251 481637-0 or send an email. We will get back to you straight away.
You can find details about what personal data we store and who has access to it in our data protection policy.

I consent to my personal data being stored and processed in line with the data protection policy and I consent my personal data being processed by Dermatest GmbH in order to answer my contact or information request.

I can withdraw my consent at any time with immediate effect.