Skin corrosion testing
OECD TG 431
For this test 3D human reconstructed skin models are incubated with the test material for 3 minutes and for one hour. After exposure, models are rinsed and vitality is assessed by MTT-test (colorimetric assay). If remaining vitality is > than 50% (3 min exposure) and >15 % (1h exposure) the test material is classified “non-corrosive”.
|Mean tissue viability (expressed as % of negative control)||Prediction|
|3 min < 50 %||corrosive|
|3 min > 50 % and 1 hour < 15 %||corrosive|
|3 min > 50 % and 1 hour > 15 %||non-corrosive|
The Test is not designed to distinguish between sub-categories of corrosivity. However, 70 % of the chemicals that are corrosive in vivo after 3 minutes exposure already, are also corrosive in the skin model after 3 minutes exposure. The test is not designed to predict skin irritation potential.