Skin corrosion testing
OECD TG 431
For this test, 3D reconstructed human skin models are incubated with the test material for 3 minutes and for one hour. After exposure, models are rinsed and viability is assessed using the MTT test (colorimetric assay). If remaining viability is > 50% (3 min exposure) and > 15% (1h exposure) the test material is classified “non-corrosive”.
|Mean tissue viability (expressed as % of negative control)||Prediction|
|3 min < 50%||corrosive|
|3 min > 50% and 1 hour < 15%||corrosive|
|3 min > 50% and 1 hour > 15%||non-corrosive|
The test is not designed to distinguish between sub-categories of corrosivity. However, 70% of the chemicals that are already corrosive in vivo after 3 minutes of exposure are also corrosive in the skin model after 3 minutes of exposure. The test is not designed to predict skin irritation potential.