Vitality, cell renewal and anti-aging effects
- Product tolerability, cytotoxicity
- Irritation
- Corrosion
- Eye irritation
- Phototoxicity
- Tissue and cell vitality
- Basal keratinocyte cell renewal/proliferation (fluorescent and KI-67 staining
- Expression of “anti-aging” markers of importance (collagens, elastin, hyaluronic acid synthase, etc.)
- Tissue differentiation, differentiation and cornification disorders
- Wound healing efficacy
- Skin barrier assessment (TEER and Lucifer yellow staining)
- Protection and regeneration approaches
- UVA and UVB irradiation studies with assessment of skin damage and quantification of CPDs (cyclopyrimidine dimer, the most common lesion after serious UVB radiation)
- ROS (reactive oxygen species) detection and quantification